Wetenschappelijke publicaties over Algen en Bacteriën in 2016

A. M. Acuña, R. Kana, M. Gwizdala, J. J. Snellenburg, P. van Alphen, B. van Oort, D. Kirilovsky, R. van Grondelle, I. H. M. van Stokkum (2016) A method to decompose spectral changes in Synechocystis PCC 6803 during light-induced state transitions, Photosynthesis Research 130(1-3): 237-249 view pubication>


S. Andreas Angermayr, A. D. Van Der Woude, D. Correddu, R. Kern, M. Hagemann, K. J. Hellingwerf (2016) Chirality matters: Synthesis and consumption of the D-enantiomer of lactic acid by Synechocystis sp. strain PCC6803, Applied and Environmental Microbiology 82(4): 1295-1304 view paper>


S. A. Angermayr, P. Van Alphen, D. Hasdemir, G. J. Kramer, M. Igbal, R. van Grondelle, H. C. J. Hoefsloot, Y.H. Choi,K. J. Hellingwerf (2016) Culturing Synechocystis sp. Strain PCC 6803 with N2 and CO2 in a Diel Regime Reveals Multiphase Glycogen Dynamics with Low Maintenance Costs., Applied and Environmental Microbiology 82: 4180 –4189. view paper>


R. Becker, S. Amirjalayer, P. Li, S. Woutersen,J. N. Reek (2016) An iron-iron hydrogenase mimic with appended electron reservoir for efficient proton reduction in aqueous media, Sci Adv 2(1): e1501014 view paper >


G. Benvenuti, P.P. Lamers, G. Breuer, R. Bosma, A. Cerar, R. H. Wijffels, M. Barbosa (2016) Microalgal TAG production strategies: why batch beats repeated-batch, Biotechnology for Biofuels 9(64) view paper >


P. Boelen, A. van Mastrigt, H. H. van de Bovenkamp, H. J. Heeres,A. G. J. Buma (2016) Growth phase significantly decreases the DHA-to-EPA ratio in marine microalgae, Aquaculture International: 1-11 view paper>


I. T. D. Cabanelas, D. M. M. Kleinegris, R. H. Wijffels,M. J. Barbosa (2016) Repeated nitrogen starvation doesn't affect lipid productivity of Chlorococcum littorale, Bioresource Technology 219: 576-582 View paper>


Q. Chen, J. B. van der Steen, H. L. Dekker, S. Ganapathy, W. J. de Grip, K. J. Hellingwerf (2016) Expression of holo-proteorhodopsin in Synechocystis sp. PCC 6803, Metabolic Engineering 35: 83-94 view paper >


W. Du, J. A. Jongbloets, H. Pineda Hernández, F. J. Bruggeman, K. J. Hellingwerf, F. Branco dos Santos (2016) Photonfluxostat: A method for light-limited batch cultivation of cyanobacteria at different, yet constant, growth rates, Algal Research 20: 118-125 view paper >


T. De Mooij, G. de Vries, C. Latsos, R. H. Wijffels, M. Janssen (2016) Impact of light color on photobioreactor productivity, Algal Research 15: 32-42 view paper >


I. Polukhina, R. Fristed, E. Dinc, P. Cardol, R. Croce (2016) Carbon supply and photoacclimation crosstalk in the green alga Chlamydomonas 4 reinhardtii, Plant Physiology 172 no. 3 1494-1505 view paper >


J. Ruiz, G. Olivieri, J. De Vree, R. Bosma, P. Willems, J. H. Reith, M. H. M. Eppink, D. M. M. Kleinegris, R. H. Wijffels,M. J. Barbosa (2016) Towards industrial products from microalgae, Energy and Environmental Science 9(10): 3036-3043 view paper >


P. Savakis, X. Tan, C. Qiao, K. Song, X. Lu, K. J. Hellingwerf, F. B. dos Santos (2016) Slr1670 from Synechocystis sp. PCC 6803 Is required for the re-assimilation of the osmolyte glucosylglycerol, Frontiers in Microbiology 7:1350, view paper>


Joris J Snellenburg, L. M. Wlodarczyk, J.P. Dekker, R. Van Grondelle, Ivo HM Van Stokkum (2016) (in Press) A model for the 77 K excited state dynamics in Chlamydomonas reinhardtii in state 1 and state 2, Biochimica et Biophysica Acta - Bioenergetics view paper>


C. Ünlü, I. Polukhina, H. van Amerongen (2016) Origin of pronounced differences in 77 K fluorescence of the green alga Chlamydomonas reinhardtii in state 1 and 2, Eur Biophys J. 45: 209-2017 view paper >


 Wetenschappelijke publicaties over Algen en Bacteriën in 2015

Acuña, A. M., J. J. Snellenburg, M. Gwizdala, D. Kirilovsky, R. van Grondelle and I. H. M. van Stokkum (2015). Resolving the contribution of the uncoupled phycobilisomes to cyanobacterial pulse-amplitude modulated (PAM) fluorometry signals. Photosynthesis Research. view article >


O. Borirak, L. J. De Koning, A. D. Van Der Woude, H. C. J. Hoefsloot, H. L. Dekker, W. Roseboom, C. G. De Koster, K. J. Hellingwerf (2015) Quantitative proteomics analysis of an ethanol- and a lactate-producing mutant strain of Synechocystis sp. PCC6803, Biotechnology for Biofuels 8(1) view paper >


I. T. D. Cabanelas, C. Fernandes, D. M. M. Kleinegris, R. H. Wijffels, M. J. Barbosa (2015) Cell diameter doesn't affect lipid productivity of Chlorococcum littorale, Algal Research: 333-341 view paper >


Chukhutsina, V., L. Bersanini, E. M. Aro and H. Van Amerongen (2015). Cyanobacterial flv4-2 operon-encoded proteins optimize light harvesting and charge separation in photosystem II. Molecular Plant 8(5): 747-761. view article >


Ganapathy, S., O. Bechau, H. Venselaar, S. Frölich, J.B. van der Steen, Q. Chen, S. Radwan, J. Lugtenburg, Pascal van Alphen and Klaas J. Hellingwerf, H.J.M. de Groot and W.J. de Grip (2015). Modulation of spectral properties and pump activity of proteorhodopsins by retinal analogues. Biochemical Journal 467(2): 333-343. view article >


P. Hammar, S. A. Angermayr, S. L. Sjostrom, J. Van Der Meer, K. J. Hellingwerf, E. P. Hudson, H. N. Joensson (2015) Single-cell screening of photosynthetic growth and lactate production by cyanobacteria, Biotechnology for Biofuels 8(1) view paper >


Le Quiniou, C., L. Tian, B. Drop, E. Wientjes, I. H. M. Van Stokkum, B. Van Oort and R. Croce (2015). PSI-LHCI of Chlamydomonas reinhardtii: Increasing the absorption cross section without losing efficiency. Biochimica et Biophysica Acta - Bioenergetics 1847(4-5): 458-467. view article >


Maarleveld, T. R., M.T. Wortel, B. G. Olivier, B. Teusink and F. J. Bruggeman (2015). Interplay between Constraints, Objectives, and Optimality for Genome-Scale Stoichiometric Models. Plos Computational Biology: 21. view article >


Savakis, P.E., X. Tan, W. Du, F. Branco dos Santos, X. Lu and Pascal van Alphen and Klaas J. Hellingwerf (2015). Photosynthetic production of glycerol by a recombinant cyanobacterium. Journal of Biotechnology 195: 46-51. view article >


P. Savakis, K. J. Hellingwerf (2015) Engineering cyanobacteria for direct biofuel production from CO2, Current Opinion in Biotechnology 33: 8-14 view paper >


R. M. Schuurmans, P. Van Alphen, J. M. Schuurmans, H. C. P. Matthijs,K. J. Hellingwerf (2015) Comparison of the photosynthetic yield of cyanobacteria and green algae: Different methods give different answers, PLoS ONE 10(9): 17 view paper>


A. Thurotte, R. L. Igual, A. Wilson, L. Comolet, C. B. de Carbon, F. Xiao,D. Kirilovsky (2015) Regulation of orange carotenoid protein activity in cyanobacterial photoprotection, Plant Physiology 169(1): 737-747 view paper >


Van Oort, B., R. Van Grondelle and I. H. M. Van Stokkum (2015). A hidden state in light-harvesting complex II revealed by multipulse spectroscopy. Journal of Physical Chemistry B 119(16): 5184-5193. view article >


J. H. De Vree, R. Bosma, M. Janssen, M. J. Barbosa,R. H. Wijffels (2015) Comparison of four outdoor pilot-scale photobioreactors, Biotechnology for Biofuels 8(1) view paper >


P. Van Alphen, K. J. Hellingwerf (2015) Sustained circadian rhythms in continuous light in Synechocystis sp. PCC6803 growing in a well-controlled photobioreactor, PLoS ONE 10(6) view paper>


 Wlodarczyk, L. M., J. J. Snellenburg, J. A. Ihalainen, R. Van Grondelle, I. H. M. Van Stokkum and J. P. Dekker (2015). Functional rearrangement of the light-harvesting antenna upon state transitions in a green alga. Biophysical Journal 108(2): 261-271. view article >


Wetenschappelijke publicaties over Algen en Bacteriën in 2014

S. A. Angermayr, A. D. Van Der Woude, D. Correddu, A. Vreugdenhil, V. Verrone ,K. J. Hellingwerf (2014) Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803, Biotechnology for Biofuels 7(1) view publication >


Anink-Groenen, L.c.M., J. Matysik, P.J. Verschure and F. J. Bruggeman (2014). Mechanistic stochastic model of histone modification pattern formation. Epigenetics & chromatin 7(30): 16. view article >


Benvenuti, G., R. Bosma, M. Cuaresma, M. Janssen, M. J. Barbosa and R. H. Wijffels (2014). Selecting microalgae with high lipid productivity and photosynthetic activity under nitrogen starvation. Journal of Applied Phycology. August 2015, Volume 27, Issue 4, pp 1425-1431. view article >


Bosma, R., J. H. de Vree, P. M. Slegers, M. Janssen, R. H. Wijffels and M. J. Barbosa (2014). Design and construction of the microalgal pilot facility AlgaePARC. Algal Research 6(PB): 160-169. view article >


Cabanelas, I. T. D., M. V. D. Zwart, D. M. M. Kleinegris, M. J. Barbosa and R. H. Wijffels (2014). Rapid method to screen and sort lipid accumulating microalgae. Bioresource Technology. view article >


Chukhutsina, V. U., C. Büchel and H. Van Amerongen (2014). Disentangling two non-photochemical quenching processes in Cyclotella meneghiniana by spectrally-resolved picosecond fluorescence at 77 K. Biochimica et Biophysica Acta - Bioenergetics 1837(6): 899-907. view article >


De Mooij, T., M. Janssen, O. Cerezo-Chinarro, J. H. Mussgnug, O. Kruse, M. Ballottari, R. Bassi, S. Bujaldon, F. A. Wollman and R. H. Wijffels (2014). Antenna size reduction as a strategy to increase biomass productivity: a great potential not yet realized. Journal of Applied Phycology. view article >


De Respinis, S., V. Monnin, V. Girard, M. Welker, M. Arsac, B. Cellière, G. Durand, P. P. Bosshard, C. Farina, M. Passera, A. Van Belkum, O. Petrini and M. Tonolla (2014). Matrix-assisted laser desorption ionization-time of flight (MALDITOF) mass spectrometry using the Vitek MS system for rapid and accurate identification of dermatophytes on solid cultures. Journal of Clinical Microbiology 52(12): 4286-4292. view article >


Drop, B., M. Webber-Birungi, S. K. N. Yadav, A. Filipowicz-Szymanska, F. Fusetti, E. J. Boekema and R. Croce (2014). Light-harvesting complex II (LHCII) and its supramolecular organization in Chlamydomonas reinhardtii. Biochimica et Biophysica Acta - Bioenergetics 1837(1): 63-72 view article>


Giera, W., S. Szewczyk, M. D. McConnell, J. Snellenburg, K. E. Redding, R. Van Grondelle and K. Gibasiewicz (2014). Excitation dynamics in Photosystem i from Chlamydomonas reinhardtii. Comparative studies of isolated complexes and whole cells. Biochimica et Biophysica Acta - Bioenergetics 1837(10): 1756-1768. view article >


Maarleveld, T. R., J. Boele, F. J. Bruggeman and B. Teusink (2014). A data integration and visualization resource for the metabolic network of Synechocystis sp. PCC 6803. Plant Physiology 164(3): 1111-1121 view article>


Schuurmans, M.R., J. Merijn Schuurmans, M. Bekker, J. C. Kromkamp, H. C. P. Matthijs and K. J. Hellingwerf (2014). The redox potential of the plastoquinone pool of the cyanobacterium synechocystis species strain PCC 6803 is under strict homeostatic control. Plant Physiology 165(1): 463-475 view article>


Van der Woude, A. D., S. A. Angermayr, V. Puthan Veetil, A. Osnato and K. J. Hellingwerf (2014). Carbon sink removal: Increased photosynthetic production of lactic acid by Synechocystis sp. PCC6803 in a glycogen storage mutant. Journal of Biotechnology 184: 100-102. view article >


H. Wulfhorst, L. E. Franken, T. Wessinghage, E. J. Boekema, M. M. Nowaczyk (2014) The 5 kDa protein NdhP is essential for stable NDH-1L assembly in Thermosynechococcus elongatus, PLoS ONE 9(8) view paper>


Wetenschappelijke publicaties van voor 2014

Wetenschappelijke publicaties Algen en Bacteriën in 2014

S. A. Angermayr, A. D. Van Der Woude, D. Correddu, A. Vreugdenhil, V. Verrone ,K. J. Hellingwerf (2014) Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803, Biotechnology for Biofuels 7(1) view publication >


Anink-Groenen, L.c.M., J. Matysik, P.J. Verschure and F. J. Bruggeman (2014). Mechanistic stochastic model of histone modification pattern formation. Epigenetics & chromatin 7(30): 16. view article >


Benvenuti, G., R. Bosma, M. Cuaresma, M. Janssen, M. J. Barbosa and R. H. Wijffels (2014). Selecting microalgae with high lipid productivity and photosynthetic activity under nitrogen starvation. Journal of Applied Phycology. August 2015, Volume 27, Issue 4, pp 1425-1431. view article >


Bosma, R., J. H. de Vree, P. M. Slegers, M. Janssen, R. H. Wijffels and M. J. Barbosa (2014). Design and construction of the microalgal pilot facility AlgaePARC. Algal Research 6(PB): 160-169. view article >


Cabanelas, I. T. D., M. V. D. Zwart, D. M. M. Kleinegris, M. J. Barbosa and R. H. Wijffels (2014). Rapid method to screen and sort lipid accumulating microalgae. Bioresource Technology. view article >


Chukhutsina, V. U., C. Büchel and H. Van Amerongen (2014). Disentangling two non-photochemical quenching processes in Cyclotella meneghiniana by spectrally-resolved picosecond fluorescence at 77 K. Biochimica et Biophysica Acta - Bioenergetics 1837(6): 899-907. view article >


De Mooij, T., M. Janssen, O. Cerezo-Chinarro, J. H. Mussgnug, O. Kruse, M. Ballottari, R. Bassi, S. Bujaldon, F. A. Wollman and R. H. Wijffels (2014). Antenna size reduction as a strategy to increase biomass productivity: a great potential not yet realized. Journal of Applied Phycology. view article >


De Respinis, S., V. Monnin, V. Girard, M. Welker, M. Arsac, B. Cellière, G. Durand, P. P. Bosshard, C. Farina, M. Passera, A. Van Belkum, O. Petrini and M. Tonolla (2014). Matrix-assisted laser desorption ionization-time of flight (MALDITOF) mass spectrometry using the Vitek MS system for rapid and accurate identification of dermatophytes on solid cultures. Journal of Clinical Microbiology 52(12): 4286-4292. view article >


Drop, B., M. Webber-Birungi, S. K. N. Yadav, A. Filipowicz-Szymanska, F. Fusetti, E. J. Boekema and R. Croce (2014). Light-harvesting complex II (LHCII) and its supramolecular organization in Chlamydomonas reinhardtii. Biochimica et Biophysica Acta - Bioenergetics 1837(1): 63-72 view article>


Giera, W., S. Szewczyk, M. D. McConnell, J. Snellenburg, K. E. Redding, R. Van Grondelle and K. Gibasiewicz (2014). Excitation dynamics in Photosystem i from Chlamydomonas reinhardtii. Comparative studies of isolated complexes and whole cells. Biochimica et Biophysica Acta - Bioenergetics 1837(10): 1756-1768. view article >


Maarleveld, T. R., J. Boele, F. J. Bruggeman and B. Teusink (2014). A data integration and visualization resource for the metabolic network of Synechocystis sp. PCC 6803. Plant Physiology 164(3): 1111-1121 view article>


Schuurmans, M.R., J. Merijn Schuurmans, M. Bekker, J. C. Kromkamp, H. C. P. Matthijs and K. J. Hellingwerf (2014). The redox potential of the plastoquinone pool of the cyanobacterium synechocystis species strain PCC 6803 is under strict homeostatic control. Plant Physiology 165(1): 463-475 view article>


Van der Woude, A. D., S. A. Angermayr, V. Puthan Veetil, A. Osnato and K. J. Hellingwerf (2014). Carbon sink removal: Increased photosynthetic production of lactic acid by Synechocystis sp. PCC6803 in a glycogen storage mutant. Journal of Biotechnology 184: 100-102. view article >


H. Wulfhorst, L. E. Franken, T. Wessinghage, E. J. Boekema, M. M. Nowaczyk (2014) The 5 kDa protein NdhP is essential for stable NDH-1L assembly in Thermosynechococcus elongatus, PLoS ONE 9(8) view paper>


Wetenschappelijke publicaties algen en bacteriën van voor 2014

Proefschriften

Batch and repeated-batch oil production by microalgae

G. Benvenuti, Wageningen University, 2016

view dissertation >


Outdoor microalgae production

J.  De Vree, Wageningen University, 2016

view dissertation >


The fatter the better: Selecting microalgae cells for outdoor lipid production

I. Dominguez Teles, Wageningen University, 2016

request copy of dissertation >


Antenna size reduction in microalgae mass culture

T. De Mooij, Wageningen University, 2016

view dissertation >


Physiological and genetic studies towards biofuel production in cyanobacteria

R.M. Schuurmans, University of Amsterdam, 2016

view dissertation >


 

Photosynthetic polyol production

P.E. Savakis, University of Amsterdam, 2016

view dissertation >


 Light harvesting, light adaptation and photoprotection in aquatic photosynthesis studies by time-resolved fluorescence spectroscopy.

Chukhutsina, V.U. (2015), Light harvesting, light adaptation and photoprotection in aquatic photosynthesis studies by time-resolved fluorescence spectroscopy. Wageningen University.

Aquatic photosynthetic organisms unavoidably experience light fluctuations that vary in amplitude, duration and origin, compromising their photosynthetic efficiency. Weather conditions and underwater flow cause continuous changes in irradiance to which the organisms have to adapt. Many light>adaptation strategies of photosynthetic organisms, such as light acclimation, photoprotection and state transitions are still not well understood. In this thesis, time>resolved fluorescence spectroscopy is used to obtain insight into the response of diatoms and cyanobacteria, both aquatic photosynthetic organisms, to changing light conditions.

The full thesis can be downloaded here (PDF, 62 Mb).


Fluxes and Fluctuations in Biochemical Models

Promovendus Timo Maarleveld van het Centrum Wiskunde & Informatica (CWI) heeft een computermodel ontwikkeld van de stofwisseling van de cyanobacterie. Geïnspireerd door het navigatiesysteem TomTom doopte Timo zijn model 'de TimoTimo'. Dit model maakt biologische computersimulaties sneller en nauwkeuriger.

De cyanobacterie is interessant, vanwege zijn potentie als “cellulaire fabriek” bij de productie van biobrandstoffen. Met zijnTimoTimo berekent Maarleveld bijvoorbeeld de meest efficiënte biologische route voor het produceren van een biobrandstof. Er zijn doorgaans meerdere biologische routes – en combinaties daarvan – die de productie van een biobrandstof optimaliseren. Dit geeft de optimale oplossingsruimte: hoe via verschillende biologische routes maximaal rendement uit de cyanobacterie gehaald kan worden.

Het werk van Maarleveld is gefinancierd door BioSolar Cells. De resultaten laten zien dat wiskunde en informatica een onmisbaar onderdeel gaan worden van onderzoek naar complexe biologische systemen.

Meer informatie over het proefschrift is te vinden op de website van de VU.


Synthetic biology of cyanobacterial cell factories.

Angermayr, S. A. (2014), University of Amsterdam - FNWI: Swammerdam Institute for Life Sciences (SILS)

In the field of microbial biotechnology rational design approaches are employed for the generation of microbial cells with desired functions, such as the ability to produce precursor molecules for biofuels or bioplastics. In essence, that is the introduction of a (new) biosynthetic pathway into a microbial cell to create a ‘microbial catalyst’. Utilizing cyanobacteria as production hosts has the potential to contribute to a bio-based economy. Cyanobacteria are prokaryotes that perform oxygenic photosynthesis. By ‘re-programming’ their metabolic network their photosynthetic metabolism can be used to synthesize valuable products from CO2, sunlight and water, O2 being the only by-product. Compared to traditional biofuel production this direct production would bypass the need to initially synthesize complex ‘biomass’ which has to be broken down again in later steps.

Key to the work presented in this thesis is the optimization of synthetic pathways, which are introduced into the cyanobacterium Synechocystis sp. PCC 6803 by means of genetic engineering. The carbon metabolism, originating from the fixation of CO2, is ‘tapped’ for the production of carbon-based compounds such as lactic acid, butanediol, and ethanol. The introduced biosynthetic pathways, which are originating from chemotrophic organisms, result in a trade-off between production and growth due to the ‘re-channeling’ of the carbon flux of the metabolism. Undoubtedly, different factors contribute to the success of a well-engineered cyanobacterial production strain. General knowledge about regulatory networks and also experience with metabolic engineering of cyanobacteria is still at the beginning.


Overige publicaties Algen en Bacteriën

H. C. Chen, D. Montesarchio, K. J. Hellingwerf, Direct Conversion: 'Artificial Photosynthesis with Cyanobacteria, Artificial photosynthesis,  Amsterdam, Elsevier, 79: 43-62, 2016


Cyanobacteria, the only bacteria that can carry out oxygenic, (ie, plant-type) photosynthesis, can be engineered with the methods of synthetic biology so that they acquire the ability to convert CO2 directly into biofuel and/or commodity chemicals. In such an approach one bypasses the formation of the entire complex set of (macro)molecules that jointly form biomass. This approach has become known as ‘direct conversion’ (of CO2 and sunlight into biofuel) and has been shown to be feasible for several products already, even upon significant scaleup. Here we explain this concept of ‘direct conversion’ through natural photosynthesis and discuss its limitations and potential further improvement.


R.M. Schuurmans, H.C.P. Matthijs, L.J. Stal, K. J. Hellingwerf, Cyanobacterial cellulose synthesis in the light of the photanol concept, CYANOBACTERIA: AN ECONOMIC PERSPECTIVE. : 181-195, January 2014


Klaas J. Hellingwerf, P van Alphen, Harvesting sunlight with cyanobacteria and algae for sustainable production in a bio-based economy. , Algae for a bio-based society (R. van Grondelle and R.E. Croce, eds), Ch 28, Taylor & Francis, New York (USA). 2014


P. P. Lamers, R. Buiter,W. P. M. Hoekstra. Algen: de groene belofte Ed., Den Haag, Stichting Bio-Wetenschappen en Maatschappij, 2013


Doorzoek de website